Ogo nri Alpha-Galactosidase kacha mma CAS 9025-35-8 Alpha-Galactosidase ntụ ntụ
Nkọwa ngwaahịa
α-galactosidase bụ enzyme nke ezinụlọ glycoside hydrolase ma na-etinye aka na hydrolysis nke njikọ galactosedic. A na-ewebata akụrụngwa anụ ahụ na kemịkal nke enzymes n'okpuru:
1. Njirimara anụ ahụ: ịdị arọ nke molecular: Ụdị ihe omimi nke α-galactosidase sitere na 35-100 kDa. Nkwụsi ike pH: Ọ nwere nkwụsi ike dị mma n'okpuru ọnọdụ acidic na nnọpụiche, na pH kwesịrị ekwesị na-abụkarị n'etiti 4.0-7.0.
2.Temperature nkwụsi ike: α-galactosidase nwere ezigbo nkwụsi ike na uru pH kwesịrị ekwesị, na-emekarị na 45-60 ° C.
3.Substrate specificity: α-galactosidase bụ isi na-akwalite hydrolysis nke α-galactosedic bonds wee wepụta α-galactose jikọrọ galactose site na mkpụrụ. Ngwakọta α-galactoside ndị a na-ahụkarị gụnyere fructose, stachyose, galactooligosaccharides, na raffinose dimers.
4.Inhibitors na accelerators: α-galactosidase ọrụ nwere ike imetụta ụfọdụ bekee: Inhibitors: Ụfọdụ metal ion (dị ka lead, cadmium, wdg) na ụfọdụ chemical reagents (dị ka arọ metal chelators) nwere ike igbochi ọrụ nke α- galactosidase.
5.Promoters: Ụfọdụ ion ígwè (dị ka magnesium, potassium, wdg) na ụfọdụ ogige (dị ka dimethyl sulfoxide) nwere ike ime ka ọrụ α-galactosidase dịkwuo elu.
Ọrụ
α-Galactosidase bụ enzyme nke isi ọrụ ya bụ hydrolyze njikọ α-galactosidase ma bepụ otu α-galactosyl na agbụ carbon iji mepụta mkpụrụ ndụ α-galactose n'efu. Ọrụ α-galactosidase na-egosipụtakarị n'akụkụ ndị a:
1.Na-enyere aka igbari galactose n'ime nri: Akwukwo nri, mkpo, na ọka nwere ọkwa alfa-galactose dị elu, shuga na-esiri ụfọdụ ndị ike ịgbari. Alfa-galactosidase nwere ike inye aka imebi alfa-galactose na nri ma kwalite mgbaze na absorption ya. Nke a dị mkpa karịsịa maka ndị na-enwe mmetụta nke alfa-galactose ma ọ bụ na-enwe nsogbu nke lactose.
2.Igbochi flatulence na irighiri nri: N'oge mgbari mmadụ, ọ bụrụ na α-galactose enweghị ike ire ere nke ọma, ọ ga-abanye na colon ma gbaa ya site na nje bacteria na-emepụta gas na eriri afọ, na-akpata flatulence na afọ ntachi. Alfa-galactosidase nwere ike inye aka kwatuo alfa-galactose ma belata omume nke mmeghachi omume ọjọọ ndị a.
3.Na-akwalite uto nke probiotics: Alpha-galactosidase nwere ike ịkwalite uto nke probiotics na eriri afọ. Ihe nje bacteria ndị a bara uru na-enyere aka ịnọgide na-enwe ahụ ike nke eriri afọ ma na-edozi microbiome. Site na imebi alfa-galactose na nri, alfa-galactose na-enye ume na nri probiotics kwesịrị itolite.
4.Applications na nhazi nri: Alpha-galactosidase na-ejikwa ya na ụlọ ọrụ nhazi nri, karịsịa na mmepụta nke ngwaahịa soy. Agwa nwere nnukwu alfa-galactose. Iji alfa-galactosidase mee ihe nwere ike ibelata ọdịnaya nke alfa-galactose na agwa ma melite udidi na uto nke nri. N'ikwu okwu n'ozuzu, α-galactosidase na-arụ ọrụ site na hydrolyzing α-galactosidase bond. Ọrụ ya gụnyere inye aka ịgbari galactose na nri, igbochi gas na mgbaze, na-akwalite uto nke probiotics na ntinye ya na nhazi nri.
Ngwa
Alfa-galactosidase bụ enzyme eji eme ihe n'ụzọ bụ isi na mpaghara dịka nri edoziri na mmepụta biofuel. Ndị a bụ ngwa ya na ụlọ ọrụ dị iche iche:
1.Food Industry: α-galactosidase nwere ike iji na nhazi ngwaahịa soy, dị ka mmiri ara ehi soy, tofu, wdg. Nke a bụ n'ihi na ụfọdụ agwa nwere alfa-galactose, shuga nke na-esiri ahụ ike ịgbari ma nwee ike dị mfe. na-akpata bloating na ahụ erughị ala. Alfa-galactosidase nwere ike imebi shuga ndị a na-esiri ike ịgbari ma nyere ahụ aka ịgbari ma banye ha nke ọma.
Ụlọ ọrụ 2.Feed: N'ime anụ ụlọ, nri aminoglycoside na-abụkarị ọgaranya na α-galactose. Ịgbakwunye α-galactosidase na-eri nri nwere ike inyere ụmụ anụmanụ aka igbari sugars ndị a ma melite arụmọrụ ntinye nri na ịrụ ọrụ uto anụmanụ.
3.Biofuel mmepụta: Alpha-galactosidase nwere ike ịrụ ọrụ na mmepụta biofuel. N'oge ntụgharị nke biomass ka ọ bụrụ biofuels, ụfọdụ polysaccharides fọdụrụnụ (dị ka galactose na oligosaccharides) nwere ike ibelata arụmọrụ gbaa ụka. Ịtinye α-galactosidase nwere ike inye aka nbibi nke polysaccharides ndị a, na-eme ka nrụpụta biomass sie ike na mmepụta biofuel.
Ụlọ ọrụ 4.Sugar: N'oge usoro mmepụta shuga nke sucrose na beet sugar, polysaccharides fọdụrụ na bagasse na beet pulp na-ezutekarị. Ịtinye alfa-galactosidase na-eme ka nkwụsị nke polysaccharides ndị a dịkwuo elu, na-amụba mkpụrụ na arụmọrụ nke usoro mmepụta shuga.
5.Pharmaceutical ubi: Alpha-galactosidase na-ejikwa na ụfọdụ ọgwụ ule na ọgwụgwọ. Dịka ọmụmaatụ, na ụfọdụ ọrịa mkpụrụ ndụ ihe nketa na-adịghị ahụkebe, ndị ọrịa enweghị ọrụ alpha-galactosidase, na-eduga na mkpokọta lipid na mgbaàmà ndị yiri ya. N'okwu a, ịgbakwunye exogenous α-galactosidase nwere ike inye aka mebie lipids gbakọtara ma belata mgbaàmà ọrịa.
Ngwaahịa ndị emetụtara:
Ụlọ ọrụ Newgreen na-enyekwa Enzymes dị ka ndị a:
Nri nri bromelain | Bromelain ≥ 100,000 u/g |
Nri ọkwa alkaline protease | Alkaline protease ≥ 200,000 u/g |
Nri ọkwa papain | Papain ≥ 100,000 u/g |
Nri nri laccase | Laccase ≥ 10,000 u/L |
Ụdị nri acid protease APRL | Acid protease ≥ 150,000 u/g |
Cellobiase ọkwa nri | Cellobiase ≥1000 u/ml |
Nri nri dextran enzyme | Dextran enzyme ≥ 25,000 u/ml |
Nri nri lipase | Lipases ≥ 100,000 u/g |
Protease na-anọpụ iche ọkwa nri | Protease na-anọpụ iche ≥ 50,000 u/g |
Glutamine transaminase nke ọkwa nri | Glutamine transaminase≥1000 u/g |
Nri nri pectin lyase | Pectin lyase ≥600 u/ml |
pectinase ọkwa nri (mmiri 60K) | Pectinase ≥ 60,000 u/ml |
Catalase ọkwa nri | Catalase ≥ 400,000 u/ml |
Ọkwa nri glucose oxidase | Glucose oxidase ≥ 10,000 u/g |
Nri nri alfa-amylase (na-eguzogide okpomọkụ dị elu) | Okpomọkụ dị elu α-amylase ≥ 150,000 u/ml |
Nri nri alfa-amylase (okpomọkụ dị ala) Ụdị AAL | Ọkpụkpọ okpomọkụ alfa-amylase ≥3000 u/ml |
Alfa-acetyllactate decarboxylase ọkwa nri | α-acetyllactate decarboxylase ≥2000u/ml |
Nri-ọkwa β-amylase (mmiri 700,000) | β-amylase ≥ 700,000 u/ml |
Ụdị nri β-glucanase BGS | β-glucanase ≥ 140,000 u/g |
Protease ọkwa nri (ụdị endo-cut) | Protease (ụdị ịkpụ) ≥25u/ml |
Ụdị nri xylanase XYS | Xylanase ≥ 280,000 u/g |
Ọkwa nri xylanase (acid 60K) | Xylanase ≥ 60,000 u/g |
Ụdị nri glucose amylase GAL | Enzyme na-eme ka ahụ sie ike≥260,000 u/ml |
Pullulanase ọkwa nri (mmiri 2000) | Pullulanase ≥2000 u/ml |
Cellulase ọkwa nri | CMC≥ 11,000 u/g |
Cellulase ọkwa nri (ihe mejupụtara ya bụ 5000) | CMC≥5000 u/g |
Ọkwa nri alkaline protease (ụdị etinye uche na-arụ ọrụ dị elu) | Ọrụ protease alkaline ≥ 450,000 u/g |
Amylase ọkwa nri (ihe siri ike 100,000) | Glucose amylase ọrụ ≥ 100,000 u/g |
Protease acid ọkwa nri (ihe siri ike 50,000) | Ọrụ protease acid ≥ 50,000 u/g |
Protease na-anọpụ iche ọkwa nri (ụdị etinye uche dị elu) | Ọrụ protease na-anọpụ iche ≥ 110,000 u/g |